JAG1 anticorps (AA 110-125)

N° du produit ABIN372790

Cet anticorps Lapin Polyclonal détecte spécifiquement JAG1 dans WB, IF, IHC (p) et EIA. Il présente une réactivité envers Humain, Souris, Rat et Chimpanzé.

Aperçu rapide pour JAG1 anticorps (AA 110-125) (ABIN372790)

Antigène: JAG1 (Jagged 1 (JAG1))

Reactivité: Humain, Souris, Rat, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JAG1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-JAG1 anticorps

Épitope: AA 110-125

Specificité: Recognizes Human Jagged-1 (JAG1).

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee, Rat and Mouse (100 % homology).Canine (81 %) and Xenopus laevis (85 %).
Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogène: Synthetic peptide corresponding to aa 110-125 of Human Jagged-1 protein.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2500-1/10000. Western Blot: 1/250-1/1000. Detects a band approximately 150 kD in size corresponding toJagged-1 in Mouse liver whole cell lysate and a 70 kD band in Human brain and kidneylysates. Immunocytochemistry. Immunofluorescence: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.53 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur JAG1

Antigène: JAG1 (Jagged 1 (JAG1))

Autre désignation: CD339 / JAG1

Sujet: Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.Synonyms: JAGL1, Jagged-1, hJ1

ID gène: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Signalisation Notch, Stem Cell Maintenance